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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
24/10/2014 |
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Actualizado : |
15/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ALEM, D.; DIAZ, P.; LEONI, C.; DE SIMONE, S.G.; CORREA, A.; OPPEZZO, P.; DALLA RIZZA, M. |
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Afiliación : |
DIEGO MARTIN ALEM GLISON, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; PAOLA ALEXANDRA DIAZ DELLAVALLE, Departamento de Ciencia y Tecnología de los Alimentos, Facultad de Ingeniería y Tecnologías, Universidad Católica del Uruguay (UCU), Montevideo, Uruguay; CAROLINA LEONI VELAZCO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
In Search of Topical Agricultural Biofungicides: Properties of the Recombinant Antimicrobial Peptide TrxAq-AMP obtained from Amaranthus quitensis. |
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Fecha de publicación : |
2014 |
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Fuente / Imprenta : |
Journal of Microbial & Biochemical Technology, 2014, v. 6, no.5, p. 268-273. |
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ISSN : |
1948-5948 |
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DOI : |
10.4172/1948-5948.1000155 |
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Idioma : |
Inglés |
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Contenido : |
ABSTRACT.
Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an
interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP
was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For
topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resistant to protease activity. Besides, this molecule is naturally stored in the seed, easy to extract and potentially produced through molecular farming. These findings encourage further biotechnological research on topical application of AMPs, especially in relation to molecule bioavailability. MenosABSTRACT.
Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an
interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP
was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For
topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resista... Presentar Todo |
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Thesagro : |
ACTIVIDAD ANTIMICROBIANA; ALTERNARIA; BIOTECNOLOGÍA; PÉPTIDOS. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/3627/1/Dalla-Rizza-M.-2014.-Jr.Microb.Biochem.-Technol.-v.65.pdf
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Marc : |
LEADER 02624naa a2200265 a 4500
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008 2014 bl uuuu u00u1 u #d
022 $a1948-5948
024 7 $a10.4172/1948-5948.1000155$2DOI
100 1 $aALEM, D.
245 $aIn Search of Topical Agricultural Biofungicides$bProperties of the Recombinant Antimicrobial Peptide TrxAq-AMP obtained from Amaranthus quitensis.$h[electronic resource]
260 $c2014
520 $aABSTRACT. Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resistant to protease activity. Besides, this molecule is naturally stored in the seed, easy to extract and potentially produced through molecular farming. These findings encourage further biotechnological research on topical application of AMPs, especially in relation to molecule bioavailability.
650 $aACTIVIDAD ANTIMICROBIANA
650 $aALTERNARIA
650 $aBIOTECNOLOGÍA
650 $aPÉPTIDOS
700 1 $aDIAZ, P.
700 1 $aLEONI, C.
700 1 $aDE SIMONE, S.G.
700 1 $aCORREA, A.
700 1 $aOPPEZZO, P.
700 1 $aDALLA RIZZA, M.
773 $tJournal of Microbial & Biochemical Technology, 2014$gv. 6, no.5, p. 268-273.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
20/06/2015 |
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Actualizado : |
20/06/2015 |
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Tipo de producción científica : |
Informes Agroclimáticos |
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Autor : |
GIMENEZ, A.; CASTAÑO, J.; FUREST, J.; AUNCHAYNA, R.; CAL, A.; TISCORNIA, G. |
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Afiliación : |
AGUSTIN EDUARDO GIMENEZ FUREST, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOSE PEDRO CASTAÑO SANCHEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOSE MARIA FUREST CROCCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ROSSINA MARIANA AUNCHAYNA REILLY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ADRIAN TABARE CAL ALVAREZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUADALUPE TISCORNIA TOSAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Informe Agroclimático 2011 - Situación a Junio. |
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Fecha de publicación : |
2011 |
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Fuente / Imprenta : |
Montevideo (Uruguay): INIA, 2011. |
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Páginas : |
4 p. |
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Idioma : |
Español |
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Palabras claves : |
AGROCLIMA; AGROCLIMATOLOGÍA; BOLETIN AGROCLIMÁTICO; CARACTERIZACIÓN AGROCLIMÁTICA; DIRECCION VIENTO; ESTACIONES AGROMETEOROLOGICAS; ESTACIONES AUTOMATICAS; ESTACIONES INIA; ESTADO DEL TIEMPO; ESTRÉS HÍDRICO; GRAFICAS AGROCLIMATICOS; GRAS; HELIOFANOGRAFO; INFORMACION SATELITAL; INUNDACIONES; LLUVIAS DIARIAS; MAXIMA; MEDIA; MINIMA; PANEL SOLAR; PERSPECTIVAS CLIMATICAS; PLUVIOMETRO; PRECIPITACION NACIONAL; PREVENCION HELADAS; PRONOSTICO; SENSOR; SIMETRICO; TANQUE A; TERMOCUPLAS; TERMOHIDROGRAFO; VARIABLES AGROCLIMATICAS; VELETA. |
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Thesagro : |
AGROCLIMATOLOGIA; CAMBIO CLIMATICO; CLIMA; CLIMATOLOGIA; ESTACIONES METEOROLOGICAS; ESTRES HIDRICO; EVAPORACION; EVAPOTRANSPIRACION; HUMEDAD; HUMEDAD RELATIVA; LLUVIA; METEOROLOGIA; PERSPECTIVAS; PLUVIOMETROS; PRONOSTICO DEL TIEMPO; SENSORES; SISTEMAS; SISTEMAS DE INFORMACION; SUELO; TEMPERATURA; TERMOMETROS. |
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Asunto categoría : |
P40 Meteorología y climatología |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/4700/1/Inf.Agr.-junio-2011.pdf
http://www.inia.uy/Publicaciones/Paginas/publicacion-2303.aspx
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Marc : |
LEADER 02093nam a2200805 a 4500
001 1052856
005 2015-06-20
008 2011 bl uuuu u0uu1 u #d
100 1 $aGIMENEZ, A.
245 $aInforme Agroclimático 2011 - Situación a Junio.$h[electronic resource]
260 $aMontevideo (Uruguay): INIA$c2011
300 $a4 p.
650 $aAGROCLIMATOLOGIA
650 $aCAMBIO CLIMATICO
650 $aCLIMA
650 $aCLIMATOLOGIA
650 $aESTACIONES METEOROLOGICAS
650 $aESTRES HIDRICO
650 $aEVAPORACION
650 $aEVAPOTRANSPIRACION
650 $aHUMEDAD
650 $aHUMEDAD RELATIVA
650 $aLLUVIA
650 $aMETEOROLOGIA
650 $aPERSPECTIVAS
650 $aPLUVIOMETROS
650 $aPRONOSTICO DEL TIEMPO
650 $aSENSORES
650 $aSISTEMAS
650 $aSISTEMAS DE INFORMACION
650 $aSUELO
650 $aTEMPERATURA
650 $aTERMOMETROS
653 $aAGROCLIMA
653 $aAGROCLIMATOLOGÍA
653 $aBOLETIN AGROCLIMÁTICO
653 $aCARACTERIZACIÓN AGROCLIMÁTICA
653 $aDIRECCION VIENTO
653 $aESTACIONES AGROMETEOROLOGICAS
653 $aESTACIONES AUTOMATICAS
653 $aESTACIONES INIA
653 $aESTADO DEL TIEMPO
653 $aESTRÉS HÍDRICO
653 $aGRAFICAS AGROCLIMATICOS
653 $aGRAS
653 $aHELIOFANOGRAFO
653 $aINFORMACION SATELITAL
653 $aINUNDACIONES
653 $aLLUVIAS DIARIAS
653 $aMAXIMA
653 $aMEDIA
653 $aMINIMA
653 $aPANEL SOLAR
653 $aPERSPECTIVAS CLIMATICAS
653 $aPLUVIOMETRO
653 $aPRECIPITACION NACIONAL
653 $aPREVENCION HELADAS
653 $aPRONOSTICO
653 $aSENSOR
653 $aSIMETRICO
653 $aTANQUE A
653 $aTERMOCUPLAS
653 $aTERMOHIDROGRAFO
653 $aVARIABLES AGROCLIMATICAS
653 $aVELETA
700 1 $aCASTAÑO, J.
700 1 $aFUREST, J.
700 1 $aAUNCHAYNA, R.
700 1 $aCAL, A.
700 1 $aTISCORNIA, G.
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